My name is Tito Jankowski, I am working on a DIYbio project to create a gel electrophoresis kit for new biologists. A good friend from high school, Scott Torborg, recommended this site to me. I’m interested in knowing if voltage will break down a watertight acrylic seal.
On a very simple level, we want to make a rectangular box, about 10"x6"x4", with no top, using laser cut acrylic pieces which we glue together. For the gel electrophoresis experiment, we fill the box with a liquid buffer and run a voltage across the buffer. We run between 100-600 volts through the buffer for about 1 hour for each experiment.
I’ve been told that at these voltages, microscopic flaws from the laser cut will propogate and compromise water tightness. Is this true? Any thoughts on this issue?
Here’s a link showing instructions for building a DIY gel electrophoresis box:
learn.genetics.utah.edu/content/ … elchamber/