What are you hoping to do with your servos?
There are a few programs that let you do some basic stuff with the Pololu servo controllers, but none are particularly stellar. For most applications I think you will want to write your own program.
If you'd like to learn a new programming language, like C for example, you can find example code around the forum and we can give you a few pointers, but if you want to stick with what you're familiar with, there's no reason not to use Matlab. Here is a simple example m-file functoin to open a serial port, move a servo, and close the serial port:
%moveServo(s,x) for Pololu serial servo controllers, using Pololu mode
%(remember to remove the blue mode-selection jumper!) absolute position
%command #4. You may need to edit the port variable in this file to match
%your com port.
%x=absolute position, 500<=x<=5500
port = 'COM3';%Edit to your com port number
ser1 = serial(port);
set(ser1, 'InputBufferSize', 2048);
set(ser1, 'BaudRate', 9600);
set(ser1, 'DataBits', 8);
set(ser1, 'Parity', 'none');
set(ser1, 'StopBits', 1);
fwrite(ser1, [128, 1, 4, s, binvec2dec(bitget(x,8:13)), binvec2dec(bitget(x,1:7))]);
I would also suggest you read through the manual carefully, but don't feel shy about asking specific questions.