If you’re not seeing the yellow user LED blink quickly three times after pressing the reset button, there could be a problem with your power supply or that the bootloader has been erased. At this point, I think we should shift our focus on getting your USBtinyISP programmer to work. There was another person on our forum that had a similar issue with trying to program the A-Star 328PB board using their USBtinyISP programmer; please follow our instructions here to configure your Arduino IDE to program the A-Star 328PB with your programmer.
- Amanda